acetyl stat3 rabbit polyclonal antibody  (Bioss)

Journal: iScience

Article Title: Role of Fyn and the interleukin-6-STAT-3-autophagy axis in sarcopenia.

doi: 10.1016/j.isci.2023.107717

Figure Lengend Snippet: Figure 1. Muscular autophagy in the tail-suspension secondary sarcopenia model (A) Selective muscle sizes from control and TS mice (n = 6 per group). Data are expressed as mean G SEM. ***p < 0.005 vs. control. (B) mRNA expression levels of atrogin-1 and MuRF-1, relative to b-actin, in the gastrocnemius muscles of TS mice (n = 3). Data are expressed as mean G SEM. ***p < 0.005 vs. control. (C) Representative immunoblots from three independent experiments with gastrocnemius muscles of control and TS mice, developed using the indicated antibodies. Signal quantification of the protein expression levels of Fyn, normalized to GAPDH (n = 3). Data are shown as mean G SEM. ***p < 0.005 vs. control. Signal quantification of the expression levels of total STAT3, normalized to GAPDH, and phosphoY705-STAT3, normalized with total STAT3 (n = 3). Data are shown as mean G SEM. ***p < 0.005 vs. control. (D) Representative H&E staining of control and TS mice. See also Figure S2.

Article Snippet: Rabbit polyclonal antibodies against STAT3 and pY705-STAT3 were obtained from Cell Signaling (Boston, MA, USA) and rabbit polyclonal antibody against pY705-STAT3 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Suspension, Control, Expressing, Muscles, Western Blot, Staining

Journal: iScience

Article Title: Role of Fyn and the interleukin-6-STAT-3-autophagy axis in sarcopenia.

doi: 10.1016/j.isci.2023.107717

Figure Lengend Snippet: Figure 2. Analysis of upstream activators of Fyn for STAT3 in C2C12 cells (A) Representative immunoblots from three independent experiments on lysates of C2C12 myoblasts and myotubes developed using the indicated antibodies. (B) C2C12 myotubes expressing either non-target or Fyn shRNA were developed using the indicated antibodies (a). Signal quantification of the expression levels of phosphoY705-STAT3 induced by IL-6 treatment, normalized to total STAT3 (n = 3). Data are expressed as mean G SEM. ***p < 0.001 vs. non-target (b). The Mann-Whitney U test was used for statistical comparison. (C) IL-6 dose dependency on phosphorylation of STAT3 in C2C12 myotubes expressing either non-target or Fyn shRNA. (D) Time dependency of IL-6 treatment on phosphorylation of STAT3 in C2C12 myotubes expressing either non-target or Fyn shRNA. The bars on each column show standard error of the mean. Abbreviations: STAT3, signal transducer and activator of transcription 3; IL-6, interleukin 6.

Article Snippet: Rabbit polyclonal antibodies against STAT3 and pY705-STAT3 were obtained from Cell Signaling (Boston, MA, USA) and rabbit polyclonal antibody against pY705-STAT3 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Expressing, shRNA, MANN-WHITNEY, Comparison, Phospho-proteomics

Journal: iScience

Article Title: Role of Fyn and the interleukin-6-STAT-3-autophagy axis in sarcopenia.

doi: 10.1016/j.isci.2023.107717

Figure Lengend Snippet: Figure 3. Fyn-dependent STAT3 regulation of autophagy (A) Representative immunoblots from three independent experiments. After 15 min of IL-6 treatment, lysates of C2C12 myotubes were immunoprecipitated with Fyn antibody and immunoblots were prepared using the indicated antibodies. (B) Representative immunoblots from three independent experiments. After 15 min of either IL-1b or IL-6 treatment, lysates of C2C12 myotubes were immunoprecipitated with total STAT3 antibody, followed by immunoblotting with Fyn. (C) Representative immunoblots from three independent experiments indicating autophagy flux in myotube lysates, determined after NH4Cl/leupeptin treatment for 2 h, followed by IL-6 treatment. Lysates were immunoblotted with the indicated antibodies (a). Signal quantification of the expression levels for LC3-II, normalized to GAPDH (n = 3) (b). Data are expressed as mean G SEM. *p < 0.05, ***p < 0.005 vs. control. The Mann-Whitney U test was used for statistical comparison. The bars on each column show standard error of the mean. Abbreviations: STAT3, signal transducer and activator of transcription; IL, interleukin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of the mean; N/L, NH4Cl/leupeptin.

Article Snippet: Rabbit polyclonal antibodies against STAT3 and pY705-STAT3 were obtained from Cell Signaling (Boston, MA, USA) and rabbit polyclonal antibody against pY705-STAT3 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Immunoprecipitation, Expressing, Control, MANN-WHITNEY, Comparison

Journal: iScience

Article Title: Role of Fyn and the interleukin-6-STAT-3-autophagy axis in sarcopenia.

doi: 10.1016/j.isci.2023.107717

Figure Lengend Snippet: Figure 4. Fyn–STAT3-dependent sarcopenia (A) Selective muscle sizes of WT and Fyn-KO mice in the control and TS groups (n = 6 per group). Data are expressed as mean G SEM. ***p < 0.005 vs. WT. (B) mRNA expression levels of atrogin-1 and MuRF-1, relative to b-actin, in the gastrocnemius muscles of WT and Fyn-KO mice with TS vs. controls (n = 3). Data are expressed as mean G SEM. ***p < 0.005 vs. WT. (C) Immunoblots of gastrocnemius muscles of WT and Fyn-KO mice (TS and control) were developed using the indicated antibodies. Signal quantification of the expression levels for phosphoY705–STAT3, normalized to total STAT3 (n = 3). Data are expressed as mean G SEM. ***p < 0.005 vs. WT. (D) mRNA expression levels of IL-6, relative to b-actin, in the gastrocnemius muscles of WT and Fyn-KO mice (TS or control) (n = 3). Data are expressed as mean G SEM. ***p < 0.005 vs. WT. The Mann–Whitney U test was used for statistical comparison. The bars on each column show standard error of the mean. Abbreviations: WT, wild-type; Fyn-KO, Fyn-knockout; SEM, standard error of the mean; STAT3, signal transducer and activator of transcription 3; IL-6, interleukin 6.

Article Snippet: Rabbit polyclonal antibodies against STAT3 and pY705-STAT3 were obtained from Cell Signaling (Boston, MA, USA) and rabbit polyclonal antibody against pY705-STAT3 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Control, Expressing, Muscles, Western Blot, MANN-WHITNEY, Comparison, Knock-Out